OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO
Abstract: Conventional
propagation of Nepenthes was difficult to do. To overcome the problems were
required alternative method such as in vitro propagation. The objective of this
research was to obtain the best treatment of BAP + NAA on shoot multiplication
of Nepenthes through in vitro culture. The research design used Randomized
Completely Design consist of seven treatments, e.g. 1) ½ MS0 (control); 2) ½ MS
+ 1 ppm BAP + 0.5 ppm NAA; 3) ½ MS + 1 ppm BAP + 1 ppm NAA; 4) ½ MS + 1.5 ppm
BAP + 0.5 ppm NAA; 5) ½ MS + 1.5 ppm BAP + 1 ppm NAA; 6) ½ MS + 2 ppm BAP + 0.5
ppm NAA dan 7) ½ MS + 2 ppm BAP + 1 ppm NAA. The parameter observed were number
of shoot, number of nodul, number of leafs, number of pitcher and number of
root. The result of this research showed that treatment of ½ MS + 1 ppm BAP + 1
ppm NAA is the best treatment compared to others. At induction stage, this
treatment can produce the number of shoot, number of nodul, and number of root
were 1.6 shoots/explant, 10.8 nodul/explant and 3.6 root/explant, respectively.
At subculture, this treatment can produce the number of shoot, number of leafs,
and number of pitcher were 5.8 shoots/explant, 12.4 leafs/explant and 5.2
pitcher/explant, respectively.
Penulis: ROSMAINA, DINNI
ARYANI
Kode Jurnal: jppertaniandd150960
