OPTIMASI METODE ISOLASI DNA PADA Jatropha spp.
Abstract: Physic nut is one of
potential plant which produces biofuel. The presence of biofuel from physic nut
is being expected could reduces the using of crude oil. It is important to do
an intensive breeding program either convensionally or molecular based to
improve new varieties of physic nut. DNA isolation is one of important step in
molecular based activity. Physic nut has high latex content which makes
isolation process become so difficult. The aim of this study is to find the
technique of DNA isolation which can reduces the contaminants. There are two
washing techniques that being used in this study, three times and five times
washing. There are also two kinds of ascorbic acid which being used in this
study, laboratory grade and 500 mg tablet vitamin C. The results showed that
isolated DNA has a good of quality and quantity. The banding pattern of
amplification clearly visible under UV light. The result showed that washing
buffer three times is enough to produce good stock DNA. Besides, 500 mg tablet
vitamin C can be used as a substitute for pure ascorbic acid in the process of
DNA isolation when the pure ascorbic acid is not available in the laboratory.
Penulis: KRISTIANTO NUGROHO,
RERENSTRADIKA T. TERRYANA, PUJI LESTARI
Kode Jurnal: jppertaniandd150958