Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii
Abstract: Optimalization
protoplast isolation of the leaves mesophyll orchid Paraphalaenopsis layockii.
The obyectives of the research was to investigate the effect of combination
treatment of sucrose concentrations and incubation time on protoplast isolation
of the leaves mesophyll orchid of Paraphalaenopsis layockii. The factorial
experiment consisted of two factors that was arranged by completely randomized
design (CRD) with ten replications. The first factor was concentrations of
sucrose which consists of three levels namely 0.4 M, 0.5 M and 0.6 M. The
second factor was incubation time which consists of four levels namely 2 hours,
4 hours, 6 hours and 8 hours. Data were analyzed using Kruskal-Wallis test
followed by Mann-Whitney test. The results showed that there was effect of
treatment for all variables observed. The highest protoplast density and number
of viable protoplasts achieved in the combined treatment of 0.6 M sucrose and 4
hours of incubation time.
Penulis: EDY SETITI WIDA
UTAMI, SUCIPTO HARIYANTO
Kode Jurnal: jppertaniandd150304
