LANGKAH SUKSES BUDIDAYA PISANG KEPOK KUNING (MUSA PARADISIAKA) BEBAS PENYAKIT MELALUI KULTUR JARINGAN SAMPAI LAPANGAN DAN PENGOLAHAN HASIL PANENNYA DI PROVINSI KALIMANTAN TIMUR
Abstrak: This research was a
solution to free desease banana kepok yellow which was decrease the banana
product in East Kalimantan. This research were devided three years and it would
be continued. The first years seedling propagation free desease through tissue
culture. The second years the seedling derived tissue culture which was planted
on the field, until harvesting fruit product. The third years processing fruit
product to several kind of industrial product. The aim of the first years
research was the highest number shoot regenerated from the explant center of
banana corm, which induction several combination treatment of plant growth
regulator Benzyl Amino Purine (BAP) and Indole Butyric Acid (IBA). This
research conducted at Tissue Culture Laboratory of Agriculture Faculty of
Mulawarman University, from March until November 2015. This research was used
Randomized Completely Block Design with two factors. The firest factor : BAP
consist 3 level consentration were : 2,5; 5 and 10 ppm and the secon factor :
IBA consist 3 level consentration were : 0; 1 and 2 ppm. So that all of
combination of plant growth regulator
were 9 treatments. Each treatments were replicated ten times. Result of this
research showed that all combination of concentration treatment of plant growth
regulator BAP and IBA could be induce the growth and differentiation of explant
center of banana corm in Murashige and Skoog (MS) media, like : inbibition,
developed tissue of explant, callusing and shooting, although the percentage
and totality was variation. The colour of callus was yellow and light green.
While the structure was hard and compact. The highest average number of shoots
induction at VII combination treatments BAP 10 ppm + IBA 0 ppm ware 3.80± 1,76
shoots/explant which was the root un completely development. So that it need
sub culture to the rooting induction media with IBA 10 ppm to be formed
completely plantlet (seedling), which could be survived on acclimatization
processing.
Penulis: Ratna Nirmala, Ratna
Shanti, Suyadi
Kode Jurnal: jppertaniandd160261
