ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT AMILOLITIK DARI INDUSTRI PENGOLAHAN PATI SAGU
Abstract: Lactic acid bacteria
(LAB) that grow on sago starch processing industry is a bacteria that can grow
in the sago-based media. Isolates which able to grow on media sago starch has
the amylolytic ability and was the potential to be used in modifying the sago
starch microbiologically. The purpose of this study was to isolate and identify
lactic acid bacteria from sago starch processing industry. Isolation and
purification of lactic acid bacteria were carried on MRS agar medium
supplemented with 0.2% CaCO3 for subsequent screening of lactic acid bacteria
that has amylolytic ability. Identification of lactic acid bacteria can be
detected by morphological include Gram stain, observation of cell shape,
catalase test and testing of gas production from glucose. Thirty-six isolates
were able to produce a clear zone on MRS agar medium supplemented with 0.2%
CaCO3, and 10 of them were amylolytic. The identification results show that the
10 isolates were Gram-positive, short-form stem cells, catalase negative. Four
isolates belong to the group of homofermentative LAB and six other isolates
were heterofermentatif LAB. Suspected lactic acid bacteria were belonged to the
genus Lactobacillus.
Keywords: sago starch,
isolation and identification, lactic acid bacteria, amylolytic ability,
Lactobacillus
Penulis: Kusumaningrum,
Yusmarini, Akhyar Ali
Kode Jurnal: jppertaniandd150742