INDUKSI KALUS PASAK BUMI (Eurycoma longifolia Jack) MELALUI EKSPLAN DAUN DAN PETIOL
Abstract: One of the problem
of Eurycoma longifolia Jack propagation was low germination percentage due to
recalcitrant seeds and slow growth of seedling from cutting propagation. To
overcome this problem is required propagation of Eurycoma longifolia via in
vitro culture. The objective of this research was to know the effect of Auxin
(2,4-D and NAA) and Cytokines (BAP and Kinetin)
on Eurycoma longifolia callus induction via leaf and petiole explants.
In this study, we used plant growth regulator of 2,4 D, NAA, BAP and Kinetin in
several levels. The observed variables
were appearing callus time, callus color and callus texture. The results of
this study showed that MS medium supplemented with 1 ppm NAA+ 1 ppm BAP was
able to induce callus formation in leaf explant for 6 months after culture.
While MS medium supplemented with 1 ppm 2,4-D, 1 ppm BAP, combination of 2,4-D
and Kinetin and combination of 2,4-D and BAP can induce callus formation from
petiole. All the callus formation has yellow color and yellow brown color. The
petiole explant that is grown in MS medium supplemented with 1 ppm BAP induced
of callus in short time (18 days after culture).
Penulis: ROSMAINA, ZULFAHMI, PROBO SUTEJO, ULFIATUN, MAISUPRATINA
Kode Jurnal: jppertaniandd150967
