INDUKSI KALUS JERUK KASTURI (CITRUS MICROCARPA) MENGGUNAKAN HORMON 2,4-D DAN BAP DENGAN METODE IN VITRO
ABSTRACT: Calamansi fruits
(Citrus microcarpa) or musk lime is one horticulture crops has been known as
beverage and food flavorings. This research aimed to determine the effect of
2,4-D and BAP on callus induction of explants calamansi fruits (Citrus
microcarpa). Parameters observed is percentage of explants, time appeared
callus, callus width, and texture of the callus. Research results and a growing
percentage of the width callus explants were analyzed by ANOVA and a further
test DMRT at 5% level. The next to appear callus timing parameters and texture
of callus analyzed in description. The result shows that a combination of
hormones D4B1 and D4B2 produces a callus emerged fastest of 3.3 days after
culture. The highest percentage of callus formation that is 100% contained in
the treatment of D2B0, D2B0.5-D4B0. The mean width of callus was highest in
treatment D4B2with a width of 0.97 cm. Embryogenic callus resulting by
treatment of D2B1, D2B2, and D3B0. However, the best a combination of hormones
is treatment D2B2 (2 mg/l, 2,4-D, dan 2 mg/l BAP) producing embryogenic callus
edvanced to suspension cultures.
Penulis: Imam Mahadi, Wan
Syafi’i, Yeni Sari
Kode Jurnal: jppertaniandd160078