INDUCED MUTATION BY GAMMA RAYS IRRADIATION TO INCREASE CHILLI RESISTANCE TO BEGOMOVIRUS
Abstract: Begomovirus
infection has a significant impact of lowering chilli yield in Indonesia. A
constraint of narrow genetic variability of chilli in Indonesia has made the
mutation breeding program as a solution worth-pursuing in increasing the
genetic variability. The objective of this study was to determine the LD50
point for each of the five irradiated chilli genotypes and the optimum dose of
gamma irradiation in inducing chilli resistance to Begomovirus and other
improved agronomical traits. The study was conducted in the Indonesian
Vegetable Research Institute (IVEGRI) at Cikole-Lembang, elevation 1,200 m
above sea level, from March to December 2013. Split plot design was used with
genotype as main factor (Kencana, Lembang-1, SSP, Tanjung 2, Seloka) and
irra-diation dosage as sub-factor (0, 200, 400, 600, 800 Gy). All treatments
were replicated three times. The results showed that LD50 points of the five
irradiated chilli genotypes were in the range of 422.64-629.68 Gy. There were
some chilli genotypes in the population of M2 that had high coefficient
variance genetic (CVG) and broad sense heritability (h2bs) value for disease
incu-bation time. This could be used as resistance parameter to Begomovirus and
improvement parameter of several agronomical traits.
Author: Redy Gaswanto, Muhamad
Syukur, Bambang Sapta Purwoko, Sri Hendrastuti Hidayat
Journal Code: jppertaniangg160025
