IDENTIFICATION OF POTENTIAL ENTOMOPATHOGENIC FUNGI OF Tetranychus kanzawai Kishida (Tetranychidae: Acarina) USING ITS-5.8s rDNA REGION AS MOLECULAR MARKER
Abstract: Fungi has been
tested as one of the potential control agents for insect pests, which raises
hopes for developing fungi as good biopesticides. The high variation within
fungi species made taxonomic identification procedures more complex, thus
molecular identification techniques are needed in addition to traditional
morphological characteristics currently used as primary methods to classify
fungi species. The objective of this research was to identify the species of
the most pathogenic fungi to Tetranychus kanzawai Kishida using RAPD-PCR. The internal transcribed spacer of 5.8s rDNA
(ITS-5.8s rDNA) sequence of these fungal isolates were amplified using two sets
of universal primers for ITS and then analyzed. Molecular identification showed
that these isolates had a higher of similarity to Metarhizium anisopliae than
Metarhizium flavoviride.
Author: Yayan Sanjaya,
Virginia R. Ocampo, Barbara L. Caoili
Journal Code: jppertaniangg160026
