GENOMIC DNA EXTRACTION METHOD FROM MATURE LEAF OF LAI (Durio kutejensis Becc.)
Abstract: Lai (Durio
kutejensis Becc.) is an indigeneous germplasm of Kalimantan which has some
superiorities compared to its close-relative, durian (Durio zibethinus Murr.).
Genetic exploration of lai is important to support its breeding program.
According to rapid development in molecular biology, genetic exploration effort
of lai will be easier. One of significant step in any molecular biology
activities is DNA isolation to produce high quality DNA for further analysis.
Leaves of lai, as other perennial crop, contain of high concen-tration of
polysaccharides and polyphenol which will be co-extracted with the DNA. These
compounds can interfere enzymes activities in subsequent molecular analysis.
The aim of this study was to establish an optimal and effective DNA extraction
method to obtain high-quality DNA from mature leaf of lai. An established
extraction buffer and its modification were used in this study. The result
showed that modification 4 could produce high quality DNA, and was considered
to be the most effective DNA extraction method for mature leaf of lai.
Author: Fitri Handayani, Rani
Agustina Wulandari, Rudi Hari Murti
Journal Code: jppertaniangg160018
