ABSTRACT: The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) in order to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrio alginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used for PCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, dan UBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higher polymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 % and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptible groups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupers produced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fish and population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fish was 0.7032.
Penulis: St. Hidayah Triana, Mahir S. Gani dan Asmi Citra Malina
Kode Jurnal: jpperikanandd140211