ANALISIS KERAGAMAN GENETIK PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP PENYAKIT YANG DISEBABKAN BAKTERI Vibrio alginolyticus
ABSTRACT: The research was
purposed to analyse genetic similarity of tiger grouper (Epinephelus
fuscoguttatus) in order to select grouper that was resistant to Vibrio
alginolyticus. In this study, fish were challenged with Vibrio alginolyticus
and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers
were used for PCR-RAPD analysis. The results showed that only three primers of
RAPD primer (YNZ-22, UBC-456, dan UBC-457) generated high number of RAPD
fragments. The resistant group of tiger groupers generated higher polymorph
RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457
generated 82 % and 71 % of RAPD polymorph fragments from resistant group of
fish and 71% and 60 % from susceptible groups, respectively. Primer YNZ-22 is a
best genetic marker to analyse genetic similarity of tiger groupers produced
specific marker ranging from 1,2-2,0 kb. Genetic distances between the
population of resistant fish and population of susceptible fish was 0.5091 and
between individual of the resistant fish and susceptible fish was 0.7032.
Penulis: St. Hidayah Triana, Mahir S. Gani dan Asmi Citra Malina
Kode Jurnal: jpperikanandd140211
