PERBANDINGAN SIFAT FISIKAWI SELULOSA BAKTERI YANG DIPRODUKSI ISOLAT KRE-12 DENGAN METODE FERMENTASI STATIS DAN PENGGOJOGAN
Abstract: Cellulose production
in bacteria is influenced by the fermentation method, both quantitatively and
qualitatively. Bacterial cellulose produced by different fermentation methods
were compared based on the dry weight of the cellulose produced. While the
physical properties compared using SEM and XRD technique. Production of
cellulose in acetic acid bacteria strain KRE-12 with static fermentation
method, shaking 50 rpm, shaking 100 rpm and shaking150 rpm was0,49; 0,12; 0,13
and 0,10 g/100 ml, respectively. The degree of crystallinity by XRD method in
static fermentation method was 91 %, shaking fermentation 100 rpm was73 % and shaking fermentation150
rpm was72%. Static fermentation method produces bacterial cellulose in
thesheets form, while shaking fermentation produces fragmented cellulose with
predominantly spherical shape. The observation of the surface structure of
bacterial cellulose by SEM showed that the static fermentation method generates
woven densely of cellulose microfibrils. The change from a static method to
shaking fermentation causes the surface structure changes. Some changes have
been observed in which woven microfibrils become more loose and form a larger
gap between the holecellulose woven microfibrils.
Penulis: Sarkono
Kode Jurnal: jpbiologidd140180
