PENGEMBANGAN MODEL MENCIT ALERGI DENGAN PAPARAN KRONIK OVALBUMIN
ABSTRACT: Histology expression
in asthma show inflammatory processes and airway structural changes. Treatment development
in asthma needs appropriate animal models but most animal models use short term
allergen exposure. The aim of this research was to create develop allergic
mouse model with allergic inflammation and structural changes histology
expression. Eight female BALB/c mice, 6-12 weeks old, 80-100 grams were divided
into two groups. The first group were exposed to a normal saline solution where
the second group received ovalbumin. Mice were sensitized by intraperitoneal
injection of ovalbumin on day 0 and 14 and challenged by nebulization of 1%
ovalbumin three times weekly for 6 weeks. Twenty four hours after final
exposure, the mice were sacrificed by lethal cardiac puncture. The level of total
IgE in serum were measured by ELISA and IL-4 receptor expression were
examinated by immunohistochemistry. Morphometric analysis was performed to
count inflammatory cells, eosinophil, goblet cells, epithel and smooth muscle
thickness. Differences between the control and exposed mice were analyzed by
Mann-Whitney test. There were a significant differences in total IgE serum and
expression IL-4 receptor between the two groups (P < 0,05). The morphometric
analyzing also demonstrated significant differences between these two groups (P
< 0,05). Chronic ovalbumin exposure through inhalation produced allergic inflammation
and airway structural changes.
Penulis: Wisnu Barlianto,
Mohamad Slamet Chandra Kusuma, Setyawati Karyono, Karyono Mintaroem
Kode Jurnal: jpkedokterandd090135
