Outer Membrane Protein 49,4 KDA dari Porphyromonas Gingivalis Merupakan Protein Hemaglutinin dan Adhesin Terhadap Netrofil
ABSTRACT: Periodontitis is
caused mostly by P. gingivalis and it is related to acute coronary syndrome. P.
gingivalis readily invades into blood circulation and potentially induces.
Collagenolytic activity of neutrophil which result in collagen vascular
degradation lead to atherosclerotic plague rupture (APR). APR is responsible
for occurring fatal cardiovascular events such as acute myocardial infarction
(AMI). These information brought out notion concerning the adhesion interaction
of P. gingivalis with neutrophil. The aim of this study was to assess adhesion
molecule of P. gingivalis outer membrane protein (OMP) by partial
characterization took in hemagglutination assay using mice erythrocytes,
adhesion inhibition assay by gradual concentration of adhesion blocked in
neutrophil, immunologic assay using Western-blotting and immunocytochemistry.
The results showed that 49,4 kDa P.gingivalis OMP can agglutinate mice erythrocytes
and adherence to netrophil Increase concentration of OMP P. gingivalis 49,4 kDa
reduce adhesion process to netrophil This protein was recognized by the
polyclonal antibody of 49,4 kDa adhesion molecule. It can be concluded that P
gingivalis outer membrane protein is a hemagglutinin and an adhesion molecule
to netrophil
Penulis: Siti Nurul Mubarokah,
Sumarno, I Ketut Gede Muliartha
Kode Jurnal: jpkedokterandd090133
